Figure 4: Effects of chronic prenatal ethanol exposure on Wnt target proteins. Pregnant dams were chronically fed with isocaloric liquid diets containing 0%, 18%, or 37% ethanol by caloric content. Cerebella harvested from perinatal (P3), juvenile (P10), and young adolescent (P20) rats were used in duplex ELISAs to measure (A) β-catenin, (B) Cyclin D1, and (C) c-Myc expression. Results were normalized to RPLPO which was measured in the same reaction wells. Inter-group comparisons were made by two- way ANOVA (Table 3) with Tukey post-hoc tests (results shown in panels). N=6 cerebella/group with each sample contributed from a different litter.