|
Symbol |
2^-ΔCt Ethanol |
2^-ΔCt Control |
Fold Change |
P-value |
| Apc |
3.8E-01 |
4.9E-01 |
-1.29 |
0.18917 |
| Apc2 |
4.3E-02 |
6.6E-02 |
-1.54 |
0.13016 |
| Axin1 |
1.5E-02 |
2.4E-02 |
-1.55 |
0.05900 |
| Axin2 |
2.5E-02 |
4.3E-02 |
-1.76 |
0.00996 |
| Bcl9 |
3.3E-02 |
4.8E-02 |
-1.46 |
0.17779 |
| Btrc |
2.3E-02 |
4.1E-02 |
-1.75 |
0.02219 |
| Ctnnb1 |
2.3E-01 |
3.7E-01 |
-1.63 |
0.08880 |
| Ccnd1 |
8.7E-02 |
1.4E-01 |
-1.64 |
0.16283 |
| Ccnd2 |
9.1E-03 |
2.3E-02 |
-2.49 |
0.03046 |
| Ccnd3 |
2.4E-02 |
4.4E-02 |
-1.85 |
0.02811 |
| Csnk1a1 |
3.7E-01 |
4.9E-01 |
-1.34 |
0.03945 |
| Csnk1d |
1.2E-01 |
1.8E-01 |
-1.43 |
0.20033 |
| Csnk2a1 |
6.0E-02 |
9.0E-02 |
-1.50 |
0.01109 |
| Csnk2b |
9.0E-01 |
1.2E+00 |
-1.32 |
0.12559 |
| Daam1 |
2.4E-01 |
4.2E-01 |
-1.76 |
0.04677 |
| Dixdc1 |
7.4E-02 |
1.2E-01 |
-1.63 |
0.00247 |
| Dkk1 |
1.1E-03 |
1.2E-03 |
-1.13 |
0.49486 |
| Dkk3 |
1.7E-01 |
2.7E-01 |
-1.58 |
0.05506 |
| Dkk4 |
1.0E-01 |
1.7E-01 |
-1.64 |
0.02177 |
| Dvl1 |
4.4E-02 |
6.8E-02 |
-1.55 |
0.15926 |
| Dvl2 |
2.4E-02 |
3.0E-02 |
-1.29 |
0.25291 |
| Dvl3 |
4.2E-02 |
7.1E-02 |
-1.70 |
0.01509 |
| Ep300 |
6.3E-02 |
1.2E-01 |
-1.96 |
0.00332 |
| Fbxw11 |
1.5E-01 |
2.1E-01 |
-1.38 |
0.27901 |
| Fbxw2 |
6.7E-01 |
8.1E-01 |
-1.20 |
0.29673 |
| Fgf4 |
5.6E-04 |
9.9E-04 |
-1.78 |
0.05312 |
| Frzb |
5.9E-03 |
9.0E-03 |
-1.53 |
0.26720 |
| Fzd1 |
1.4E-01 |
2.0E-01 |
-1.49 |
0.12972 |
| Fzd2 |
1.9E-03 |
4.8E-03 |
-2.47 |
0.06658 |
| Fzd3 |
1.4E-01 |
2.1E-01 |
-1.55 |
0.02392 |
| Fzd4 |
7.4E-03 |
1.3E-02 |
-1.71 |
0.03654 |
| Fzd5 |
2.2E-03 |
4.6E-03 |
-2.08 |
0.16799 |
| Fzd6 |
7.0E-03 |
1.3E-02 |
-1.91 |
0.00437 |
| Fzd7 |
2.2E-02 |
2.9E-02 |
-1.28 |
0.20717 |
| Fzd9 |
2.0E-03 |
4.5E-03 |
-2.26 |
0.09042 |
| Gsk3a |
4.8E-01 |
7.1E-01 |
-1.47 |
0.07896 |
| Gsk3b |
5.6E-01 |
7.9E-01 |
-1.41 |
0.07885 |
| Jun |
4.7E-02 |
6.5E-02 |
-1.39 |
0.30477 |
| Kremen1 |
5.3E-03 |
9.0E-03 |
-1.69 |
0.01538 |
| Lef1 |
1.5E-02 |
2.2E-02 |
-1.52 |
0.07159 |
| Lrp5 |
3.2E-03 |
7.3E-03 |
-2.24 |
0.00549 |
| Lrp6 |
1.0E-01 |
1.3E-01 |
-1.26 |
0.17383 |
| Mitf |
2.0E-02 |
4.9E-02 |
-2.45 |
0.13361 |
| Myc |
2.0E-03 |
4.0E-03 |
-1.98 |
0.00666 |
| Nkd1 |
1.2E-02 |
2.6E-02 |
-2.09 |
0.21437 |
| Nkd2 |
6.8E-03 |
1.3E-02 |
-1.85 |
0.04517 |
| RGD1561440 |
2.4E-02 |
4.2E-02 |
-1.80 |
0.00311 |
| Pitx2 |
1.7E-03 |
1.7E-03 |
1.03 |
0.88505 |
| Porcn |
2.3E-01 |
2.8E-01 |
-1.24 |
0.16598 |
| Ppp2ca |
1.3E+00 |
1.8E+00 |
-1.38 |
0.12881 |
| Ppp2r1a |
5.7E-01 |
9.0E-01 |
-1.57 |
0.00621 |
| Pygo2 |
2.9E-03 |
6.3E-03 |
-2.17 |
0.02359 |
| Rhoa |
1.2E+00 |
2.1E+00 |
-1.71 |
0.00353 |
| Senp2 |
1.6E-01 |
2.6E-01 |
-1.56 |
0.02894 |
| Sfrp1 |
2.3E-02 |
5.3E-02 |
-2.30 |
0.02068 |
| Sfrp2 |
3.7E-03 |
7.7E-03 |
-2.09 |
0.19428 |
| Sfrp4 |
8.2E-04 |
1.3E-03 |
-1.56 |
0.00836 |
| Sfrp5 |
1.1E-03 |
1.6E-03 |
-1.51 |
0.05005 |
| Tcf3 |
9.0E-03 |
1.7E-02 |
-1.94 |
0.00054 |
| Tcf4 |
5.8E-01 |
7.8E-01 |
-1.33 |
0.06372 |
| Tcf7 |
2.3E-03 |
3.3E-03 |
-1.43 |
0.25535 |
| Tcfe2a |
8.1E-03 |
1.3E-02 |
-1.59 |
0.14644 |
| Tle1 |
5.8E-02 |
9.1E-02 |
-1.55 |
0.16625 |
| Tle2 |
4.1E-01 |
5.6E-01 |
-1.39 |
0.34059 |
| Wif1 |
2.8E-03 |
5.0E-03 |
-1.79 |
0.01399 |
| Wisp1 |
3.3E-03 |
4.5E-03 |
-1.34 |
0.25818 |
| Wnt1 |
4.7E-04 |
5.5E-04 |
-1.16 |
0.44400 |
| Wnt10a |
4.3E-04 |
4.8E-04 |
-1.12 |
0.67497 |
| Wnt10b |
6.0E-04 |
6.3E-04 |
-1.04 |
0.73714 |
| Wnt11 |
5.6E-03 |
1.2E-02 |
-2.20 |
0.11090 |
| Wnt2 |
1.0E-03 |
1.4E-03 |
-1.43 |
0.10151 |
| Wnt2b |
1.4E-02 |
2.1E-02 |
-1.44 |
0.02325 |
| Wnt3 |
8.4E-03 |
1.3E-02 |
-1.56 |
0.09550 |
| Wnt3a |
5.7E-04 |
8.2E-04 |
-1.42 |
0.51981 |
| Wnt4 |
5.0E-03 |
1.1E-02 |
-2.09 |
0.04808 |
| Wnt5a |
6.8E-03 |
1.8E-02 |
-2.62 |
0.00029 |
| Wnt5b |
4.4E-03 |
1.0E-02 |
-2.36 |
0.00360 |
| Wnt6 |
2.4E-03 |
3.0E-03 |
-1.27 |
0.62605 |
| Wnt7a |
7.7E-03 |
1.5E-02 |
-1.99 |
0.01008 |
| Wnt7b |
2.4E-03 |
3.8E-03 |
-1.60 |
0.37925 |
| Wnt8a |
1.6E-03 |
1.7E-03 |
-1.04 |
0.87043 |
| Wnt8b |
4.9E-03 |
7.4E-03 |
-1.51 |
0.17107 |
| Wnt9a |
1.4E-03 |
2.4E-03 |
-1.73 |
0.01723 |
| Wnt9b |
8.0E-04 |
1.4E-03 |
-1.73 |
0.52746 |
Gene expression was measured in cerebellar tissue of P35 rats in which the pregnant dams were chronically fed with isocaloric ethanol-containing (37% caloric content) or
control (0% ethanol) liquid diets from gestation day 6 through delivery. Offspring were sacrificed and cerebella were harvested on P10
(not shown) or P35. Gene expression was measured in a targeted Wnt Pathway array that included 84 genes. (See gene full names, related Wnt pathways, and functions in
Table 1). Gene expression was calculated using the DDCt method with results normalized to HPRT (which did not change with ethanol exposure or age). Fold differences
were calculated. Data from 3 sets of pooled samples (3 cerebella each; 9 total per group) were analyzed using T-tests with corrections for multiple comparisons. Values in
Red indicate significant differences (P<0.05). Values in Blue reflect statistical trends (0.05<P<0.10). |
