Protein Age Ethanol Age x Ethanol
  F-Ratio P-Value F-Ratio P-Value F-Ratio P-Value
β-Catenin 66.65 < 0.0001 5.95 0.0051 5.39 0.0012
Cyclin D1 25.78 < 0.0001 0.56 N.S. 11.93 <0.00018
c-Myc 21.48 < 0.0001 6.67 0.0029 5.10 0.0018
Duplex ELISAs were used to measure immunoreactivity. Results were normalized to large ribonuclear protein which remained stable with respect to ethanol treatment and postnatal age. Results were analyzed by Two-way ANOVA with post-hoc Tukey tests for significance. F-ratios and corresponding P-values for postnatal age, ethanol treatment and dose, and interaction between age and ethanol exposure are listed. N.S.= Not Significant
Table 3: Effects of Age and Ethanol Dose on Wnt Target Protein Expression.