|
ONCOTYPE Dx |
MAMMAPRINT |
ROTTERDAM SIGNATURE |
GENOMIC GRADE |
P53 SIGNATURE |
INVASIVINESS GENE SIGNATURE |
DEADTH FROM CANCER SIGNATURE |
PAM-50 |
| Indication |
Node-negative, ER+ or PR+, HER-2 normal. Stage I-II tumor. Identify low risk patients who may not need adjuvant chemotherapy. |
<61 years patients Node-negative, ER+ or PR+, HER-2 normal, early breast cancer. |
Node negative patients early breast cancer. Risk of metastasis. |
Early breast cancer ER positive. |
Clinical outcome |
Early breast cancer and node negative patients |
Different solid tumors |
Node negative, untreated patients. Identify risk patients. Evaluate efficacy of neoadjuvant treatment. |
| Type of assay |
21-gene recurrence score |
70-gene assay |
76-gene assay |
97-gene assay |
32-gene assay |
186-gene assay |
11-gene assay |
50-gene assay |
| Results classification |
Recurrence Score High(>31), intermedium(30≥18, low risk(<18) |
High/low risk |
High/low risk |
High/low risk Poor/good response to endocrine therapy |
High/low risk (P53 mutation vs wild type tumors) |
Good/poor prognosis |
Prognosis Treatment response Malignant clinical behavior |
ROR-C ROR-S |
| Genes evaluated |
PROLIFERATION INVASION ESTROGEN GSTM-1 REFERENCES |
GROWTH PROLIFERATION ANGIOGENESIS LOCAL INVASION SURVIVAL IN CIRCULATION INTRA-EXTRAVASATION |
CELL DEATH, CELL CYCLE AND PROLIFERATION, DNA REPLICATION AND REPAIR, IMMUNE RESPONSE, DISEASE PROGRESSION 18 GENES UNKNOWN FUNCTION |
PROLIFERATION GENES ENDOCRINE PATHWAY GENES |
PROLIFERATION TRANSCRIPTION ION TRANSPORT BREAST CANCER BIOLOGY |
TUMOROGENIC CANCER CELLS |
BMl1 ONCOGENIC PATHWAY |
LUMINAL GENES PROLIFERATION GENES CONTROL GENES |
| Type of tissue |
Formalin-fixed, paraffin-embedded |
Fresh or frozen |
Formalin-fixed, paraffin-embedded |
Fresh or frozen |
Frozen |
Fresh |
Fresh or frozen |
Fresh or frozen Formalin-fixed, paraffin-embedded |
| Molecular assay |
qRT-PCR |
DNA microarrays (Agilent) |
DNA microarrays (Affimetrix) |
Oligonucleotides microarrays (Affimetrix) |
Oligonucleotides microarrays (Affimetrix) |
Oligonucleotides microarrays (Affimetrix) |
Oligonucleotides microarrays (Affimetrix) |
DNA microarrays (Agilent) pRT-PCR |
| Prospective trial |
YES: TAILORx |
YES: MINDACT |
NO |
NO |
NO |
NO |
NO |
NO |
