| Bacteria treatment |
Phagocytes |
Bactericidal index (%) |
| Diurnal |
Nocturnal |
| |
|
EPEC |
S. pneumoniae |
EPEC |
S. pneumonia |
| PBS |
MN
PMN |
25.7±4.2
25.8±4.2 |
58.6±10.5#
58.7±13.6# |
34.2±6.5
21.1±4.8 |
46.5±10.5
59.3±5.5# |
| Serum |
MN
PMN |
53.7±4.2*
41.8±8.9* |
56.2±12.3
60.2±10.6 |
58.7±5.6*
32.4±7.5 |
60.0±16.1*
48.2±10.7 |
| Colostrum
|
MN
PMN |
54.9±6.9*
47.1±9.4* |
51.7±14.2
56.0±12.9 |
44.4±3.8*
32.3±5.0† |
62.7±8.2*
62.5±11.0# |
| Purifed SIgA |
MN
PMN |
53.5±8.1*
43.8±5.4* |
45.3±16.9
55.4±17.5 |
47.7±5.6*
32.1±6.5† |
50.0±13.0
49.0±6.3 |
Bactericidal activity by colostrum phagocytes was determined with the acridine orange method. Bacteria were opsonized with a colostrum supernatant pool, purifeid SIgA
or a normal serum pool. In controls assays, mononuclear (MN) and polimorphonuclear (PMN) cells were pre-incubated with PBS. The results represent the mean and
SD of ten experiments with cells from different individuals. *p=0.001 (F=5.11) comparing the treated groups to PBS group, considering the same collection period, kind
of cells and bacteria. #p=0.001 (F=10.92) comparing bacteria type, considering the same opsonin source, kind of cells and collection time. †p=0.048 (F=2.68) comparing
bactericidal active between the different collection time, considering the same treatment, kind of cells and bacteria. |
