Figure 1: LAMP assay performed using total DNA purified from mouse liver/ spleen homogenate, known to contain the Karp strain of Orientia. Lane 1: 100bp DNA ladder, Lane 2: positive control, Lane 3: negative control, Lane 4-6: products from LAMP reaction containing different copies (lane 4-6 has 20,000, 2,000, and 200 copies, respectively) of Orientia DNA and an overabundance (1,000 fold excess) of mouse DNA.