|
Primer Name |
Length (bp) |
Tm(°C) |
Sequence 5’- 3’ |
|
LAMP |
|
|
|
| Primer Set 1 (#3) |
|
|
|
| F3 |
22 |
58.9 |
CTATTCATATGGGTAGCTTTGG |
| B3 |
25 |
58.0 |
CCTAAATTCTCATTTAATTCTGGAG |
| FIP |
51 |
69.6 |
AGCAAAACTTATGCCTGAGTAAGAT-TTTTGACCGATGTTTAATCTTGAAGG |
| BIP |
50 |
69.7 |
CTGTACTTGAAGCAGTTGAATGCT-TTTTACATTTAACATACCACGACGAA |
| Primer Set 2 (#5) |
|
|
|
| F3 |
24 |
57.7 |
TGAAGTTATAAAAGAAGGATCTGC |
| B3 |
23 |
57.4 |
GCTTTGATCATTACTTTGTTGCT |
| FIP |
47 |
70.0 |
ACCTGTAAATCTCTTCCTGTTTTGAT-TTTTGGAATTGCTCCTGGAGA |
| BIP |
49 |
70.6 |
CTGAAAGAGAAGTTGAGCTTTTACG-TTTTACTATCCTCACCTTTGTTGG |
| qPCR |
|
|
|
| 47kDa-F |
28 |
58.8 |
AACTGATTTTATTCAAACTAATGCTGCT |
| 47kDa-R |
30 |
63.3 |
TATGCCTGAGTAAGATACGTGAATGGAATT |
