Figure 2: LSP based PCR typing of Asian strains. The Japanese strains M. ulcerans/M. shinshuense Japan 882 and M. shinshuense 753 were analysed together with M. ulcerans strains from China (ITM 980912) and Malaysia (ITM 941328), and compared with control strains of the South American haplotype (Surinam 842), a representative of the classical lineage (M. ulcerans Ghana IFIK1066089), and the patient isolate M. marinum M. Amplicons shown are (from top to down): RD9: 887 bp product covering M_MAR3536 which is deleted in ΔRD9A of the Asian InDel haplotype; RD12: 543 bp product bridging the large Asian deletion ΔRD12C; RD11: 1581 bp product bridging the Asian deletion ΔRD11A::IS2404 – the 4780 bp product reveals sequence preservation (M_MAR2557 through M_MAR2563) in other strains – strain Malaysia 941328 may have a specific genetic signature here; RD13: 968 bp product spanning genes esxB/esxA missing in the classical lineage; RD6: 183 bp product present in M. marinum and the M. ulcerans Asian InDel haplotype, but absent from the classical lineage – the 1548 bp product contains an IS2404 element carried by the M. ulcerans South American haplotype; RD7: 488 bp product specific for M. ulcerans but absent in M. marinum – a 1926 bp product is found only in the M. ulcerans classical lineage (contains a copy of IS2606), the South American haplotype carries here a secondary deletion; RD9: 507 bp product (within M_MAR3547) only present in M. marinum and the M. ulcerans Asian InDel haplotype. For positions of the RDs, see [14]. Equal amounts of DNA were applied to the DNA amplification reactions. Primer sequence information is provided in supplementary table S1.