Figure 2: The pluripotent state of hESCs is associated with active chromatin remodeling factor Brg-1 and hSNF2H. (A) High level of expression and nuclear localization of Brg-1 (green) in undifferentiated hESCs, as indicated by Oct-4 (red) expression, inside the colony, and significantly reduced immune-reactivity of differentiated cells outside the colony. (B) High level of expression and mostly nuclear localization of hSNF2H (green) in undifferentiated hESCs, as indicated by Oct-4 expression, inside the colony, and significantly reduced immune-reactivity of differentiated cells outside the colony. (C) Weak expression of Brm (green) in undifferentiated hESCs, as indicated by Oct-4 expression, inside the colony. (D) Mostly cytoplasmic localization of Mi-2 (green) in undifferentiated hESCs, as indicated by Oct-4 expression, inside the colony, and significantly reduced immune-reactivity of differentiated cells outside the colony. All cells are shown by DAPI staining (blue). Highresolution microscopic images in the bottom panel reveal cellular localization patterns.