Figure 3: The pluripotent state of hESCs is associated with p300 and HDAC1. (A) High level of expression and nuclear localization of p300 (red), and cytoplasmic localization of Tip60 (green) and Moz (red), and weak expression of HBO-1 (red) in undifferentiated hESCs, as indicated by Oct-4 (red) expression, inside the colony. (B) High level of expression and nuclear localization of HDAC1 (green), and weak expression of HDAC3 (green), the class I HDACs, in undifferentiated hESCs, as indicated by Oct-4 expression, inside the colony. (C) Mostly cytoplasmic localization of HDAC7 (green), HDAC4 (green), HDAC5 (green), and HDAC6 (green), the class II HDACs, in undifferentiated hESCs, as indicated by Oct-4 expression, inside the colony. (D) Cytoplasmic localization of SIRT1 (red), the class III HDAC, in undifferentiated hESCs inside the colony. (E) Cytoplasmic localization of SUV39H1 (red) in undifferentiated hESCs inside the colony. All cells are shown by DAPI staining (blue). High-resolution microscopic images in the bottom panel reveal cellular localization patterns.