Figure 2: Diabetic nephropathy of eNOS-/- mice after 6 months of diabetes induced by streptozotocin. (A). Glomerular morphology.WT NC: a glomerulus from a wild type non-diabetic mouse fed a normal chow; eNOS-/- DM NC shows mesangiolysis; eNOS-/- diabetic mice fed a high fat diet show severe glomerulosclerosis. (B). Immunostaining against TF and monocytes/ macrophages (MOMA-2) in a diabetic eNOS-/-mouse fed a HF diet. The left lower panel is a differential interference contrast image of the glomerulus. (C). Kidney IL-6 mRNA expression in diabetic mice. C: control; AF: anti-mouse TF neutralizing antibody AF3178 administered i.p. once at 5 weeks of STZ-induced diabates, and the animals were sacrificed for analysis 4 days later. (Adopted from reference 11 with permission).