Figure 3: Cytotoxicity of infiltrating whole T cells and CD8+ T cells isolated from the transplanted hearts. The whole T cells isolated from transplanted hearts in vehicle-treated allograft recipients showed obvious cytotoxicity against splenocytes as target cells derived from ACI rats. However, the cytotoxicity of the isolated whole T cells from FR276457-treated allograft recipients was weaker than those from vehicle-treated allograft recipients (A). The cytotoxicity of CD8+ T cells isolated from FR276457-treated allograft recipients against splenocytes from ACI rat was also weaker than those from vehicle-treated allograft recipients (B,C). In each experiment, effector cells, isolated from four allografts of vehicle-treated allograft recipients or FR276457-treated allograft recipients, were pooled and used. The studies were carried out at different E:T ratios (10 to 1 and 30 to 1 for Figure 3A and 3B, and 12.5 to 1 and 25 to 1 for Figure 3C) to confirm the activity of effector cells.