A: Cationic-exchange chromatography on HPLC (Mono S HR 5/5) of the whey protein fraction performed as described in the Materials and Methods section. Lactoferrin was identified in peak D. Other protein identified were: peak A, β-lactoglobulin; peak B, α-lactalbumin, peak C, lysozyme.
B. 12% SDS-PAGE and Immunoblotting. St Bio-Rad low-molecular-weight standard 1) 12% SDSPAGE of peak D eluted from the Mono S column. 2) Immunoblotting of peak D eluted from the Mono S column by using anti-lactoferrin antibodies.