SPOT

Mr (kDa)°

pI°

Identification*

A1

33.50

5.90

β-casein

B1

32.87

6.06

β-casein

C1

31.90

5.92

β-casein

D1

26.80

5.88

αS1-casein

E1

28.48

6.10

αS1-casein

F1

30.60

6.70

αS1-casein

G1

30.60

6.86

αS1-casein

H1

30.80

5.60

αS1-casein

I1

32.30

5.68

β-casein

° The calculation of experimental pI and molecular mass was carried out using PDQuest software Version 7.1.1 (Bio-Rad Laboratories).
* NCBI BLAST database at http://blast.ncbi.nlm.nih.gov/
Table 3: Donkey milk casein dephosphorylation experiments & Characterization of the dephosphorylated (CIP-treated) casein fraction.