Ligand

Eu-GTP

[35S]GTPγS

Agonists

 

 

Imetit

9.03±0.11

8.82±0.07

Immepi p

9.44±0.01

9.29±0.07

R(-)-α-methylhistamine

8.85±0.08

8.57±0.01

Proxyfan

8.36±0.12

  8.3±0.10

Histamine

7.55±0.07

7.46±0.04

Antagonists

 

 

GSK189254

9.90±0.11

9.06±0.02

Clobenpropit

9.00±0.02

9.28±0.06

Thioperamide

8.26±0.10

8.12±0.01

Table 1: Dose-response isotherms of ligands were analyzed by non-linear regression. Agonist potency is expressed as pEC50. The antagonist potency to inhibit Eu-GTP binding induced by 100 nM Imetit is expressed as PKB. Results are presented as means ± S.E.M. of at least three independent experiments.