HPLC 2010
Column Phenomenex luna C18 column 250×4.6
Wavelength 205 nm
Temperature 30°C
Flow rate 1.0 ml/min
Detector UV
Injection volume 10 μl
Mobile phase Acetonitrile, Water in the ratio of 60:40(v/v)
Retention time 4.76 min
Table1: Solution preparation.