Figure 5: Effect of enzyme concentration on protease activity. (A): leaf, (B): bark and (C): root. 50 μg of BSA for leaf and 25 μg of BSA for bark and root (1 mg/ml) with varying amount of protease was added. The final volume was made 200 μl by adding phosphate buffer. It was the incubated for 30 min at room temperature and direct method was performed.