Purification step
Volume (ml)
Protein concentration (mg/ml)
Total protein (mg)
Activity (nmol min
-1
ml
-1
)
Total activity (nmol min
-1
)
Specific activity (nmol min
-1
mg
-1
)
Purification factor
a
Overall yield (%)
b
Crude extract
152
2.06 ± 0.13
313.79 ± 1.33
1.494 ± 0.03
227.15 ± 4.67
0.74 ± 0.01
1
100
TCA ppt. extract
8
2.66 ± 0.56
21.29 ± 0.46
1.74 ± 0.07
13.92 ± 0.56
0.654 ± 0.03
0.88
6.13
20-80% ammonium sulfate ppt. extract
9
2.1 ± 1.24
17.494 ± 1.63
1.707 ± 0.01
15.36 ± 0.14
0.883 ± 0.07
1.19
6.76
Note:
a
Defined as: purification factor=(specific activity of fraction/specific activity of homogenate).
b
Defined as: overall yield=total activity of fraction/total activity of homogenate)×100.
Table 1:
Summary of protease purification of leaf.