Purification step
Volume (ml)
Protein concentration (mg/ml)
Total protein (mg)
Activity (nmol min
-1
ml
-1
)
Total activity (nmol min
-1
)
Specific activity (nmol min
-1
mg
-1
)
Purification factor
a
Overall yield (%)
b
Crude extract
148
1.02 ± 0.66
150.38 ± 3.26
0.663 ± 0.04
98.062 ± 6.49
0.653 ± 0.05
1
100
TCA ppt. extract
3
1.93 ± 1.28
5.784 ± 0.19
0.883 ± 0.005
2.643 ± 0.01
0.458 ± 0.01
0.7
2.7
20-80% ammonium sulfate ppt. extract
3.7
0.61 ± 0.56
2.257 ± 0.04
0.469 ± 0.002
1.336 ± 0.008
0.769 ± 0.01
1.18
1.8
Note:
a
Defined as: purification factor=(specific activity of fraction/specific activity of homogenate).
b
Defined as: overall yield=total activity of fraction/total activity of homogenate)×100.
Table 2:
Summary of protease purification of bark.