| Pair Primers |
Gradient |
Temperature,/Voltage/Time |
Results |
| 1071FW-1282RV |
10-100%, 10-40%, 20-40%, 25-35% ,40-80% and
70-90%
(each) |
60 to 70°C,
150- 220V,
4 to 6.5h. |
Never increased the difference between the mutant and wild sequence migration
(Fig. 1A) |
| 1071FW-1207RV |
| 1071FW-1255RV |
| GC107FW1-1282RV |
10-40 and 20-40%
(each) |
Better difference in the pattern migration between the sequences was at 65°C, 210V and 4h.
(see Fig. 1C) |
| GC107FW1-1207RV |
| GC107FW1-1255RV |
FW-forward, RV- reverse GC tailed oligonucleotide
|