Figure 1: Immunocytochemical localization of caveolin-1 in ram spermatozoa. Two patterns were identified: A) A restricted location in the most apical acrosome (type A); B) An extensive distribution in the acrosome and equatorial regions (type B); Magnification X1000; C) Endothelial cells, positive control. Magnification X600; D) Negative control without the primary antibody. Magnification X1000.E) Quantitative analysis of the stained sperm patterns: at the most apical part of the acrosome (type A), a more extensive staining on the whole acrosome (type B), and non-stained cells (N S) (mean values ± SEM; n=4).