Figure 3: Distribution of (A) cholesterol, (B) ganglioside GM1, and (C) total proteins in the fractions obtained by 2 (■) or 20 h (■) ultracentrifugation of Triton X-100 lysates in an OptiprepTM gradient. Mean values ± SEM (n=4). Significant differences in the same fractions at different ultracentrifugation times: *P<0.05; ***P<0.001.