Figure 2: Location of human Cbl mutations in the context of known structure of RING finger and helical linker region. The N-terminus of Cbl containing the TKB (4H – blue, EF – yellow, SH2 – red), linker helical region (green), and RING finger domains (orange) is shown in complex with a Zap70 peptide (purple) and the E2 ubiquitin-conjugating enzyme UbcH7 (grey). Residues that have been found to be mutated in human leukemia/myelodysplastic syndromes are shown in blue. Note that the mutations cluster in and around the binding pocket responsible for E2 interactions. (Modeled using pyMol software [http://www.pymol.org/]. 1FBV PDB file [Zheng et al. 2000] was downloaded from NCSB Protein Data Bank [http://www.rcsb.org/pdb]).