Figure 1: Effect of MTX on total protein in oviduct (A) Uterus (B) Cervix (C) Vagina (D) Ovariectomized rats. OVX: Ovariectomized; MTX: Methotrexate, LD: Low dose, HD: High Dose, LCN: Leucovorin; E2: Estradiol; P: Progesterone P<0.05, ‘a’ vs Control, ‘b’ vs OVX+MTX, ‘c’ vs OVX+MTX+LCN, ‘d’ vs OVX+E2, ‘e’ vs OVX+MTX+LCN+E2, ‘f’ vs OVX+P. Values are Mean ± SE, N=6. Note marked reduction in total protein concentrations in MTX treated group and in combination with LCN and steroids. Also, note the significant differences between treatment groups and tissues.