Figure 5: Effect of MTX on ALP in oviduct (A), uterus (B), cervix (C) and vagina (D) of ovariectomized rats. OVX: Ovariectomized; MTX: Methotrexate; LD: Low Dose; HD: High Dose; LCN: Leucovorin; E2= Estradiol; P: Progesterone; ALP= Alkaline Phosphatase P<0.05, ‘a’ vs Control, ‘b’ vs OVX+MTX, ‘c’ vs OVX+MTX+LCN, ‘d’ vs OVX+E2, ‘e’ vs OVX+MTX+LCN+E2, ‘f’ vs OVX+P. Values are Mean ± SE, N=6. Note marked reduction in ALP activity in MTX treated group and in combination with LCN and steroids. Also, note the significant differences between treatment groups and tissues.