Figure 2: Measurement of FGFR2 copy number from a dilution series of tumor versus normal genomic DNA using ddPCR. To ascertain the limits of quantifying amplifications, Kato III genomic DNA, known to harbor FGFR2 amplification, was diluted with a normal diploid genomic DNA in decreasing ratios. Data points with error bars represent the drop in FGFR2 copy number as the Kato III genomic DNA is diluted with normal genomic DNA.