Figure 1: PCR amplification of normal and mutant alleles of R282W p53 point mutations by ASO- PCR method. L1:100bp ladder. ✔ P1(patient1): L2–L3: Both normal and mutant allele amplified :P1 positive for mutation ✔ P2 (patient1): L4–L5: Only normal allele amplified :P2 negative for mutation ✔ P3(patient1): L6–L7: Both normal and mutant allele amplified :P3 positive for mutation ✔ L8: NTC Non template control