Relevant Histological Subtypes Pathway Molecular Diagnostics Potentially relevant Therapies Lung adenocarcinoma EGFR Cobas® EGFR Mutation Test (Roche, Indianapolis, IN) Therascreen EGFR PCR Kit (Qiagen, Valencia, CA) TKIs Colorectal cancer KRAS Therascreen KRAS RGQ PCR Kit (Qiagen) MEK1/MEK2 inhibitors, EGFR inhibitors Lung adenocarcinoma EML4-ALK Vysis ALK Break Apart FISH Probe Kit (Abbott Laboratories, Abbott Park, IL, USA) ALK inhibitors,  e.g. critzotinib Lung adenocarcinoma* ROS1 Vysis FISH for ROS1 rearrangement (Abbott) Crizotinib Melanoma* BRAF V600E Cobas® 4800 BRAF V600 Mutation Test (Roche, Indianapolis, IN) Vemurafenib Breast Cancer** HER2 HER2 FISH pharmDx Kit (Dako, Carpinteria, CA) HercepTest immunocytochemical assay (Dako) Trastuzumab *Tumors at advanced stage should be tested at time of diagnosis, or at time of recurrence of progression in patients who originally presented with lower stage disease but were not previously tested. **Tumors should be tested in when trastuzumab treatment is being considered

Table 1: Molecular diagnostics in selected cancer types.