Figure 2: NsPEFs eliminate N1-S1-Luc tumors determined by luminescence 7-10 days after treatment. -Tumors were initiated as indicated in the legend to Figure 1. Seven days after initiation tumors were treated with 1000 pulses (1000X) with durations of 100 ns and electric fields at 50 kV/cm at 1 Hz. On the day of treatment (day 0) and 7 and 10 days after treatment tumor bioluminescence was determined on the same animals using non-invasive in vivo imaging with the IVIS Spectrum (Caliper Life Sciences, Xenogene). One sham-treated tumor and one typical rat with a treated tumor are shown.