Figure 5: NsPEF treatment destroys HCC tumors and induces infiltration of immune cells. – N1-S1-Luc HCC tumors were initiated as described in the legend to Figure 1 and treated as described in the legend to Figure 2. Then, 14 days after treatment rats were humanely euthanized and liver tissue was taken from normal liver removed from a distant untreated lobe, from shamtreated HCC tumor and from HCC tumor treated with nsPEFs. Tissues were washed in PBS, fixed in 10% Neutral Buffered Formalin (NBF), followed by paraffin embedded, sectioning and H&E staining. Images were visualized using a Nikon Eclipse 50i microscope (Nikon, Tokyo, Japan) equipped with a 10X/0.25 numerical aperture, and acquired using a Go-5 camera (QImaging, Surrey, Canada) and QCapture Pro 6.0 software.