Figure 4: Anastomosis procedures. The donor heart was harvested (a). The ascending aortic artery of the donor heart was drawn over the end of the CA and fixed to the arterial cuff with a circular ligature of 7-0 silk. Similarly, by pulling on the MPA, a 22-gauge cuff fixed to the jugular vein was inserted into the MPA and fixed with a circular 7-0 silk ligature for taping (b and c).