Storing duration Temperature ACON ® One Step Test Device Positive n (%) Negative n (%) 2 weeks 37°C 48 (96) 2 (4) 45°C 48 (96) 2 (4) 50°C 48 (96) 2 (4) 4 weeks 37°C 48(96) 2 (4) 45°C 47 (94) 3 (6) 50°C 46 (92) 4 (8) 8 weeks 37°C 46 (92) 4 (8) 45°C 45 (90) 5 (10) 50°C 42 (84) 8 (16)

Table 2: The effect of temperature and storage time on testing of 50 HIV positive DBS samples with ACON ® rapid test.