Figure 3: NsPEFs eliminate tumors for as long as 7 weeks. – N1-S1-Luc tumors were initiated and treated with 1000 pulses with 100 ns durations and 50 kV/cm at 1 Hz. In this typical trial, 7 sham and 8 treated rats were treated. After treatment, tumors were followed and analyzed by ultrasound. When sham treated tumors reach 1.5 cm, they were humanely euthanized according to our IACUC approved protocol. Forty nine days after treatment the experiment was terminated and animals were humanely euthanized. All surviving animals had been treated and were tumor free. The designation 1000X indicates that tumors were pulsed 1000 times.