A: Higher expression of TRH-DE mRNA in induced neuronal cells from canine pulp CD31- SP cells compared to unfractionated total pulp cells, pulp stem/ progenitor CD31- side population (SP) cells, CD105+ cells. Data are expressed as means ± SD at 4 determinations (*P<0.005).
B,C: Immunohistochemical analysis of localization of TRH-DE (B) and PGP9.5 (C) in human normal pulp tisssue. TRH-DE protein is expressed in neuronal processes.
D-F: Immunohistochemical and in situ hybridization analyses in human normal pulp tisssue by confocal microscopy. PGP9.5 (D), TRH-DE mRNA (E) and Merge (F). TRH-DE mRNA expression overlapped PGP 9.5 in neuronal process.
G: H&E staining in the adjacent section to (D-F). Arrows indicate neuronal processes.
