Figure 3: Regulation of TRH-DE mRNA in human pulp cells. Enhanced expression of TRH-DE mRNA 24 hours after treatment with 3×10-6 M neuropeptide Y (NPY), and significantly lower expression with 10-7 M thyrotropin-releasing hormone (TRH) in canine unfractionated total pulp cells.Little effect by 10-7 M α Calcitonin Gene Related Peptide (CGRP). Data are expressed as means ± SD derived from three independent experiments (*P<0.05, **P<0.001). The experiment was repeated three times, and one representative experiment is presented.