A,B: Complete pulp regeneration 28 days after autologous transplantation of CD31- SP cells with SDF-1. H&E staining.Low power (A). High power regenerated pulp showing stellate-like pulp cells and vessel (v) (B).
C: Immunostaining with BS-1 lectin. Arrow indicates vessel.
D: Odontoblast-like cells extending their process (arrows) into dentinal tubules, showing characteristics of dentin-pulp complex. H&E staining.
E: PGP9.5 immunohistochemistry. Arrow indicates nerve fibers.
F-K: Confocal microscopic analysis of colocalization of PGP9.5 (F, I) with TRHDE mRNA(G, J) by immunohistochemistry and in situ hybridization. Merge image (H, K). TRH-DE mRNA expression overlapped PGP9.5 positive nerve fibers in regenerated pulp tissue (I-K) as normal pulp tissue (F-H).
L: Similar mRNA expression of TRH-DE in regenerated pulp tissue to that in normal pulp, and significant difference from that in periodontal ligament. Data are expressed as means ± SD at 4 determinations (*P<0.01).
