Control, No additions, 0 min; Control, No additions, 60 min; MSU, 0 min; MSU, 60 min. A representative of one of the 3 MSC samples obtained for this study is shown

Figure 5: MSU Does Not Increase the Number of TUNEL-Positive Chondrocytes in Macroaggregate Pellet Cultures Initiated from MSCs. A chondrocyte macroaggregate pellet culture initiated from MSCs (BMDMSC- C) was fixed in formalin and embedded in paraffin. A 5 μm histologic cross-section was prepared from the experimental group that had been incubated for 60 min with MSU (see Materials and Methods for details). Following deparaffinization, the cross-sections were processed for detection of TUNEL-positive chondrocytes and then the chondrocytes were stained with DAPI. A merged DAPI/TUNEL stained cross-section is shown.