Figure 2: Cell properties after induction of differentiation. A: gene expression profile of the Pitt (light gray boxes) and NMI (dark gray boxes) cells. B: typical size difference of the differentiated micromasses (left: Pittsburgh cells, right: NMI cells). C: sGAG content in culture supernatant for two donors (Pitt, light gray boxes; NMI, dark gray boxes). Both Pitt and NMI cell preparations differentiate equally well, there is a slight tendency for the NMI cells towards higher Col2 expression (* = significant, p<0.05, t-test if normality and equal variance tests are passed, if not rank sum test was performed). Bars = 500 μm.