Gene

abbreviation

forward primer

reverse primer

efficiency

Reference sequence

tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, zeta polypeptide

YWHAZ

ACTTTTGGTACATTGTGGCTTCAA

CCGCCAGGACAAACCAGTAT

1.89

NM_003406

collagen, type I, alpha 2

COL1A2

GCTGGCAGCCAGTTTGAATATAAT

CAGGCGCATGAAGGCAAGT

1.96

NM_000089

collagen, type II, alpha 1

COL2A1

AGAGGTATAATGATAAGGATGTGTGGAAG

GTCGTCGCAGAGGACAGTCC

1.94

NM_001844

aggrecan

ACAN

TGCATTCCACGAAGCTAACCTT

GACGCCTCGCCTTCTTGAA

1.98

NM_013227

melanoma inhibitory activity

MIA

CCCAGTAGCATTGTCCGAGA

GGCAGTAGAAATCCCATTTGTCT

1.91

NM_006533

alkaline phosphatase

ALP

TTCCCACGTCTTCACATTTGG

TTGCCATACAGGATGGCAGTG

1.95

NM_000478

collagen, type X, alpha 1

COL10A1

CACGCAGAATCCATCTGAGAAT

CGTTCAGCGTAAAACACTCCAT

1.96

NM_000493