Cell source

Cell status
at harvest

Purification/
enrichment

Plating

Expansion

Appearance after expansion

NMI protocol

bone marrow aspirate from femur shaft

in suspension

density gradient centrifugation

mononuclear cell fraction

60 % DMEM low glucose, 40 % MCDB-201,
2 % FCS,
1x ITS+1,
1.1 mM ascorbate,

0.05 µM dexamethasone,
10 ng/ml PDGF-BB,
10 ng/ml EGF
[ Reyes 2001]

heterogeneous

Pittsburgh protocol

trabecular bone marrow from femoral head

adherent

none

whole cell fraction

a-MEM,
10 % FBS (MSC qualified),
1 ng/ml FGF-2

homogeneous