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Figure 4: Histochemical staining of Vigna radiata seeds for superoxide accumulation. Seeds were germinated for 12 h in the medium containing ABA (1 mM), AgNO3 (AG, 5 mM), ethylene (ETH, 0.05 mM), GA (1 mM) and paclobutrazole (PAC, 1 mM) along with a control (CON) followed by staining in NBT solution for localization of superoxide producing zones. Photographs are of representative seeds showing the average effect for respective treatments. |