Figure 5: In-gel assay for NADPH oxidase (NOX) activity profile of axes from seeds of Vigna radiata subjected to treatments with hormones and their inhibitors. Seeds were germinated for 12 h in the medium containing (A) GA (1 mM), paclobutrazole (PAC, 1 mM), ABA (1 mM), fluridone (Flu, 1 mM) and (B) ethylene (ETH, 0.05 mM), AgNO3 (AG, 5 mM) along with control (Con). Axes were extracted with Triton X100 for membrane proteins which was then run in native gel followed by NBT staining for NOX activity specific bands.