Substrate Relative activity (%) Vmax (pkat.mg-1) Km (µM) Kcat/Km (mM-1.min-1)
Quercetin 100 500 128.5 1587.4
Caffeic acid 45.3 175.4 190.1 594.8
Luteolin 19.3      
Myricetin 3.6      
Tricetin 2.7      
5-HFA 2.1      
*The standard enzyme assay was used as described in the Methods section.
Table 2: Enzymatic activity of NtOMT2 with a number of selected substrates*.