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Figure 1: The human IL-2 expression cassette used to transform maize plants. The IL-2 gene was cloned in frame with C-myc and 6X HIS tags to allow for easy protein extraction. RbcS1 P: ribulose bisphosphate carboxylase promoter; 1.3 ER Secretion: signal peptide for targeting of the rhIL-2 into the ER; MCS: multiple cloning site, c-myc: c-termain myc epitope; 6X HIS: Six histidine tag; KDEL: RbcS1 P: ribulose bisphosphate carboxylase promoter (Figure modified from Matakas et al.) [1]. |