The current research on sequencing of the Mycobacterium tuberculosis genome holds assure for the development of new vaccines and the design of new drugs (Prachee Chakhaiyar and Hasnain, 2004) The functions for genomic sequences of hypothetical proteins are unknown because this is a protein whose being has been predicted (Edward Eisenstein et al, 2000). In depth learn of function prediction on such proteins will offer opportunity for novel applications and help the researchers to Identify new drug molecules for tuberculosis. Mycobacterium tuberculosis organism has totally 3887 number of proteins. In these proteins 1985 hypothetical proteins were present Out of the 250 hypothetical proteins taken for this work. All hypothetical proteins were analyzed for function prediction using Bioinformatics web tools such as BLAST, INTERPROSCAN, PFAM and COGs. The results indicates 100% confidence for only 86 proteins, with 75% confidence for 92 proteins and some proteins function could not be predicted with much confidence (unknown function).

Complete genome sequence of pathogenic bacteria Mycobacterium tuberculosis sequences were downloaded from the PIR Database (http://pir.georgetown.edu/) and NCBI Database (www.ncbi.nlm.nih.gov/). In complete genome sequence of Mycobacterium tuberculosis, 1985 hypothetical proteins were present. Only 250 hypothetical proteins of genome sequence were analyzed and then downloaded from the site (http://www.ncbi.nih.gov/genomes/lproks.cgi). Finally genomics sequences of each protein were submitted to functions prediction web tools such as NCBI-BLAST2 ((Wendy Baker et al, 2000)), INTERPROSCAN (Zdobnov and Rolf Apweiler, 2001), PFAM (Bateman et al, 2002) and COG (Roman et al, 2000). The confidence level can be measured on the basis of above tools.

There is rising death of humans worldwide by reason of tuberculosis (Smith et al, 2004). Central goal of Bioinformatics is recognized as the major area of research to determining protein functions from their genomic sequences and to develop personalized medicine. Functional annotations of genomic sequences for hypothetical proteins are of major importance in providing insights into their molecular functions and will help in the identification of new drugs for tuberculosis. Table 1 shows the functional genomics of Mycobacterium tuberculosis by using tools such as BLAST, INTERPROSCAN, PFAM and COG. Mycobacterium tuberculosis organism has totally 3887 number of proteins. In this 3887 proteins 1985 were hypothetical proteins from which 250 hypothetical proteins were retrieved for this study. Those hypothetical proteins were submitted to above tools, which help to determine the confidence level. Among 250 proteins, 244 proteins only were obtained the function such as DEHYDROGENASES/REDUCTASE, HYDROLASES, LUCIFERASES & METHYL TRANSFERASES were in more in number.Table 2