Time (min)       Acetonitrile (%)         Water (%)       Flow rate (ml/min.) 0-3.64 1 99 0.4 3.64-4.04 19 81 0.4 4.04-4.93 1 99 0.4 4.93-5.00 1 99 0.4

Table 2: gradient used for separation in UPLC (Ultra Performance Liquid Chromatography).