| 
      ° The calculation of  experimental pI and Mr was carried out using PDQuest software  Version 7.1.1 (Bio-Rad Laboratories).
        | Spot | Mr (kDa)° | pI° | N-terminal sequence | Protein identification* | Accession no.* |  
        | A | 33.74 | 4.63 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | B | 33.54 | 4.72 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | C | 33.10 | 4.82 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | D | 33.54 | 4,92 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | E | 31.66 | 4.68 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | F | 31.48 | 4,80 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | G | 32,15 | 4,88 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | H | 31.15 | 4,95 | RKEELNVSS | b-casein | P86273 (donkey) |  
        | I | 31.20 | 5.15 | RPKLPHRPE | αs1-casein | P86272(donkey) |  
        | J | 31.14 | 5.23 | RPKLPHRPE | αs1-casein | P86272(donkey) |  
        | L | 31.14 | 5.36 | RPKLPHRPE | αs1-casein | P86272 (donkey) |  
        | M | 28.26 | 5.08 | RPKLPHRPE | αs1-casein | P86272 (donkey) |  
        | N | 27.24 | 4.92 | RPKLPHRPE | αs1-casein | P86272 (donkey) |  
        | O | 21.90 | 4.46  | TNIPQTMQDLDLQEV | β-lactoglobulin   | P13613 (donkey)  |  
        | P | 20.0 | 4.40  | TNIPQTMQDLDLQEV | β-lactoglobulin  | P13613 (donkey) |  
        | Q | 20.58 | 4.12  | TNIPQTMQDLDLQEV | β-lactoglobulin | P13613 (donkey |  
        | R | 13.04 | 4.76  | KQFTKHELSQVLKSM | α-lactalbumin | P28546 (donkey) |  
        | S | 12.98 | 5.26  | KQFTKHELSQVLKSM | α-lactalbumin | P28546donkey) |  
        | T | 14.5 | 9.40  | KVFSKHELAHKL | lysozyme  | P11375 (donkey) |  
        | U | 66.3 | 6.62  | DTHKSEIAHRFND | serum albumin | Q5XLE4 (donkey) |  
        | V | 79.0 | 9.70  | APRKSVRW | lactoferrin | O77811 (horse) |  * NCBI BLAST database  at http://blast.ncbi.nlm.nih.gov/
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