Figure 2: Effect of pH on protease activity of ammonium sulfate extract. (A): leaf, (B): bark and (C): root extract. Reaction mixture contained 25 μg BSA (1 mg/ml), 12 μl enzyme (28.56 μg) for leaf, 19 μl enzyme (11.78 μg) for bark and 22 μl enzyme (13.42 μg) for root were taken and volume was maintained to 200 μl buffers of different pH. After 30 min incubation at room temperature direct method was performed.