Purification step
Volume (ml)
Protein concentration (mg/ml)
Total protein (mg)
Activity (nmol min-1 ml
-1
)
Total activity (nmol min
-1
)
Specific activity (nmol min
-1
mg
-1
)
Purification factor
a
Overall yield (%)
b
Crude extract
43
1.94 ± 1.2
83.33 ± 1.72
1.096 ± 0.01
4712 ± 0.49
0.566 ± 0.01
1
100
TCA ppt. extract
3
1.35 ± 0.58
4.05 ± 0.09
0.986 ± 0.01
2.957 ± 0.04
0.731 ± 0.01
1.29
6.28
20-80% ammonium sulfate ppt. extract
6.2
0.57 ± 0.59
3.52 ± 0.07
0.695 ± 0.01
4.213 ± 0.04
1.225 ± 0.04
2.16
8.94
Note:
a
Defined as: purification factor=(specific activity of fraction/specific activity of homogenate).
b
Defined as: overall yield=total activity of fraction/total activity of homogenate)×100.
Table 3:
Summary of protease purification of root.