| Eluants |
Amount of solvent (in ml) |
No. of collected beakers |
| 100% n-hexane |
300 |
1-3 |
| 25% Petroleum ether in hexane |
100 |
4 |
| 50% Petroleum ether in hexane |
100 |
5 |
| 100% Petroleum ether |
200 |
6-7 |
| 2.5% Ethyl acetate in petroleum ether |
100 |
8 |
| 5% Ethyl acetate in petroleum ether |
300 |
9-11 |
| 7.5% Ethyl acetate in petroleum ether |
100 |
12 |
| 10% Ethyl acetate in petroleum ether |
300 |
13-15 |
| 12.5% Ethyl acetate in petroleum ether |
100 |
16 |
| 15% Ethyl acetate in petroleum ether |
100 |
17 |
| 20% Ethyl acetate in petroleum ether |
100 |
18 |
| 25% Ethyl acetate in petroleum ether |
100 |
19 |
| 30% Ethyl acetate in petroleum ether |
200 |
20-21 |
| 40% Ethyl acetate in petroleum ether |
100 |
22 |
| 50% Ethyl acetate in petroleum ether |
100 |
23 |
| 60% Ethyl acetate in petroleum ether |
100 |
24 |
| 70% Ethyl acetate in petroleum ether |
100 |
25 |
| 80% Ethyl acetate in petroleum ether |
100 |
26 |
| 90% Ethyl acetate in petroleum ether |
100 |
27 |
| 100% Ethyl acetate |
100 |
28 |
| 5% Methanol in ethyl acetate |
200 |
29-30 |
| 15% Methanol in ethyl acetate |
100 |
31 |
| 50% Methanol in ethyl acetate |
200 |
32-33 |
| 100% Methanol wash |
100 |
34 |
The fractions were combined on the basis of their preliminary TLC examination
to give combined fractions A, B, C, D, E, F (Table.2). Each combined fraction was
evaporated to dryness under reduced pressure.